laser scanning confocal microscopy Search Results


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Evident Corporation laser scanning confocal microscopy
Polarization phenotype of RAW 264.7 cells cultured on the membranes in LPS‐induced inflammatory microenvironment. A) IF staining of macrophages for phenotypic markers, iNOS (red), Arg‐1 (green), and DAPI (blue) using <t>LSCM</t> (scale bar, 20 µm). B) Representative peak plots of CD80+ and CD206+ macrophages detected by flow cytometry and C) quantification data. D) qRT‐PCR analysis of transcription levels of macrophage polarization‐related genes ( iNOS , TNF‐α , CD206 , and Arg‐1 ) in RAW 264.7 cells. E) The levels of IL‐10 and TNF‐α secretion as quantified by ELISA. n = 3 per group. Data are expressed as mean ± SD. Ns, no significance. * p < 0.05, ** p < 0.01, *** p < 0.001.
Laser Scanning Confocal Microscopy, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Evident Corporation confocal laser scanning microscopy
Polarization phenotype of RAW 264.7 cells cultured on the membranes in LPS‐induced inflammatory microenvironment. A) IF staining of macrophages for phenotypic markers, iNOS (red), Arg‐1 (green), and DAPI (blue) using <t>LSCM</t> (scale bar, 20 µm). B) Representative peak plots of CD80+ and CD206+ macrophages detected by flow cytometry and C) quantification data. D) qRT‐PCR analysis of transcription levels of macrophage polarization‐related genes ( iNOS , TNF‐α , CD206 , and Arg‐1 ) in RAW 264.7 cells. E) The levels of IL‐10 and TNF‐α secretion as quantified by ELISA. n = 3 per group. Data are expressed as mean ± SD. Ns, no significance. * p < 0.05, ** p < 0.01, *** p < 0.001.
Confocal Laser Scanning Microscopy, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Evident Corporation immunofluorescence laser scanning confocal microscope
Polarization phenotype of RAW 264.7 cells cultured on the membranes in LPS‐induced inflammatory microenvironment. A) IF staining of macrophages for phenotypic markers, iNOS (red), Arg‐1 (green), and DAPI (blue) using <t>LSCM</t> (scale bar, 20 µm). B) Representative peak plots of CD80+ and CD206+ macrophages detected by flow cytometry and C) quantification data. D) qRT‐PCR analysis of transcription levels of macrophage polarization‐related genes ( iNOS , TNF‐α , CD206 , and Arg‐1 ) in RAW 264.7 cells. E) The levels of IL‐10 and TNF‐α secretion as quantified by ELISA. n = 3 per group. Data are expressed as mean ± SD. Ns, no significance. * p < 0.05, ** p < 0.01, *** p < 0.001.
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Evident Corporation laser confocal scanning fluorescence microscopy
Polarization phenotype of RAW 264.7 cells cultured on the membranes in LPS‐induced inflammatory microenvironment. A) IF staining of macrophages for phenotypic markers, iNOS (red), Arg‐1 (green), and DAPI (blue) using <t>LSCM</t> (scale bar, 20 µm). B) Representative peak plots of CD80+ and CD206+ macrophages detected by flow cytometry and C) quantification data. D) qRT‐PCR analysis of transcription levels of macrophage polarization‐related genes ( iNOS , TNF‐α , CD206 , and Arg‐1 ) in RAW 264.7 cells. E) The levels of IL‐10 and TNF‐α secretion as quantified by ELISA. n = 3 per group. Data are expressed as mean ± SD. Ns, no significance. * p < 0.05, ** p < 0.01, *** p < 0.001.
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Evident Corporation laser scanning confocal microscopy lscm
Polarization phenotype of RAW 264.7 cells cultured on the membranes in LPS‐induced inflammatory microenvironment. A) IF staining of macrophages for phenotypic markers, iNOS (red), Arg‐1 (green), and DAPI (blue) using <t>LSCM</t> (scale bar, 20 µm). B) Representative peak plots of CD80+ and CD206+ macrophages detected by flow cytometry and C) quantification data. D) qRT‐PCR analysis of transcription levels of macrophage polarization‐related genes ( iNOS , TNF‐α , CD206 , and Arg‐1 ) in RAW 264.7 cells. E) The levels of IL‐10 and TNF‐α secretion as quantified by ELISA. n = 3 per group. Data are expressed as mean ± SD. Ns, no significance. * p < 0.05, ** p < 0.01, *** p < 0.001.
Laser Scanning Confocal Microscopy Lscm, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Evident Corporation laser confocal scanning microscopy
Polarization phenotype of RAW 264.7 cells cultured on the membranes in LPS‐induced inflammatory microenvironment. A) IF staining of macrophages for phenotypic markers, iNOS (red), Arg‐1 (green), and DAPI (blue) using <t>LSCM</t> (scale bar, 20 µm). B) Representative peak plots of CD80+ and CD206+ macrophages detected by flow cytometry and C) quantification data. D) qRT‐PCR analysis of transcription levels of macrophage polarization‐related genes ( iNOS , TNF‐α , CD206 , and Arg‐1 ) in RAW 264.7 cells. E) The levels of IL‐10 and TNF‐α secretion as quantified by ELISA. n = 3 per group. Data are expressed as mean ± SD. Ns, no significance. * p < 0.05, ** p < 0.01, *** p < 0.001.
Laser Confocal Scanning Microscopy, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Evident Corporation confocal microscope
Polarization phenotype of RAW 264.7 cells cultured on the membranes in LPS‐induced inflammatory microenvironment. A) IF staining of macrophages for phenotypic markers, iNOS (red), Arg‐1 (green), and DAPI (blue) using <t>LSCM</t> (scale bar, 20 µm). B) Representative peak plots of CD80+ and CD206+ macrophages detected by flow cytometry and C) quantification data. D) qRT‐PCR analysis of transcription levels of macrophage polarization‐related genes ( iNOS , TNF‐α , CD206 , and Arg‐1 ) in RAW 264.7 cells. E) The levels of IL‐10 and TNF‐α secretion as quantified by ELISA. n = 3 per group. Data are expressed as mean ± SD. Ns, no significance. * p < 0.05, ** p < 0.01, *** p < 0.001.
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Evident Corporation fv1000 confocal laser scanning microscopy
Polarization phenotype of RAW 264.7 cells cultured on the membranes in LPS‐induced inflammatory microenvironment. A) IF staining of macrophages for phenotypic markers, iNOS (red), Arg‐1 (green), and DAPI (blue) using <t>LSCM</t> (scale bar, 20 µm). B) Representative peak plots of CD80+ and CD206+ macrophages detected by flow cytometry and C) quantification data. D) qRT‐PCR analysis of transcription levels of macrophage polarization‐related genes ( iNOS , TNF‐α , CD206 , and Arg‐1 ) in RAW 264.7 cells. E) The levels of IL‐10 and TNF‐α secretion as quantified by ELISA. n = 3 per group. Data are expressed as mean ± SD. Ns, no significance. * p < 0.05, ** p < 0.01, *** p < 0.001.
Fv1000 Confocal Laser Scanning Microscopy, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Evident Corporation ax8635 laser scanning confocal microscopy
Polarization phenotype of RAW 264.7 cells cultured on the membranes in LPS‐induced inflammatory microenvironment. A) IF staining of macrophages for phenotypic markers, iNOS (red), Arg‐1 (green), and DAPI (blue) using <t>LSCM</t> (scale bar, 20 µm). B) Representative peak plots of CD80+ and CD206+ macrophages detected by flow cytometry and C) quantification data. D) qRT‐PCR analysis of transcription levels of macrophage polarization‐related genes ( iNOS , TNF‐α , CD206 , and Arg‐1 ) in RAW 264.7 cells. E) The levels of IL‐10 and TNF‐α secretion as quantified by ELISA. n = 3 per group. Data are expressed as mean ± SD. Ns, no significance. * p < 0.05, ** p < 0.01, *** p < 0.001.
Ax8635 Laser Scanning Confocal Microscopy, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Polarization phenotype of RAW 264.7 cells cultured on the membranes in LPS‐induced inflammatory microenvironment. A) IF staining of macrophages for phenotypic markers, iNOS (red), Arg‐1 (green), and DAPI (blue) using LSCM (scale bar, 20 µm). B) Representative peak plots of CD80+ and CD206+ macrophages detected by flow cytometry and C) quantification data. D) qRT‐PCR analysis of transcription levels of macrophage polarization‐related genes ( iNOS , TNF‐α , CD206 , and Arg‐1 ) in RAW 264.7 cells. E) The levels of IL‐10 and TNF‐α secretion as quantified by ELISA. n = 3 per group. Data are expressed as mean ± SD. Ns, no significance. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Advanced Healthcare Materials

Article Title: Bredigite‐Based Bioactive Nerve Guidance Conduit for Pro‐Healing Macrophage Polarization and Peripheral Nerve Regeneration

doi: 10.1002/adhm.202302994

Figure Lengend Snippet: Polarization phenotype of RAW 264.7 cells cultured on the membranes in LPS‐induced inflammatory microenvironment. A) IF staining of macrophages for phenotypic markers, iNOS (red), Arg‐1 (green), and DAPI (blue) using LSCM (scale bar, 20 µm). B) Representative peak plots of CD80+ and CD206+ macrophages detected by flow cytometry and C) quantification data. D) qRT‐PCR analysis of transcription levels of macrophage polarization‐related genes ( iNOS , TNF‐α , CD206 , and Arg‐1 ) in RAW 264.7 cells. E) The levels of IL‐10 and TNF‐α secretion as quantified by ELISA. n = 3 per group. Data are expressed as mean ± SD. Ns, no significance. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: EdU‐labeled cells were observed via laser scanning confocal microscopy (LSCM, Olympus, Japan).

Techniques: Cell Culture, Staining, Flow Cytometry, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

Effects of polarized macrophages activated by 5% BRT/PCL membrane on biological behaviors of RSC96 cells in a co‐culture system. A) Schematic plot of RSC96 cells exposure using Transwell system. B) Longitudinal RSC96 cells migration induced by activated macrophages for 24 h (scale bar, 1 mm). C) Quantitative analysis of longitudinal migration of RSC96 cells. D) Schematic illustration of RSC96 cells treatment with conditioned culture media. E) Representative images from wound healing assays for RSC96 cells migration at 0, 12, and 24 h (scale bar, 400 µm). F) Quantitative analysis of the healing rates. G) Expression levels of myelination‐related genes ( PMP22 , NGF , and NCAM ) in RSC96 cells after co‐culture with the polarized macrophages for 3 days. H) IF staining for cytoskeletal protein F‐actin (green), neuron‐specific protein S100β (red), and nuclear dye DAPI (blue) as visualized by LSCM (scale bar, 100 µm). n = 3 per group. Data are expressed as mean ± SD. Ns, no significance. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Advanced Healthcare Materials

Article Title: Bredigite‐Based Bioactive Nerve Guidance Conduit for Pro‐Healing Macrophage Polarization and Peripheral Nerve Regeneration

doi: 10.1002/adhm.202302994

Figure Lengend Snippet: Effects of polarized macrophages activated by 5% BRT/PCL membrane on biological behaviors of RSC96 cells in a co‐culture system. A) Schematic plot of RSC96 cells exposure using Transwell system. B) Longitudinal RSC96 cells migration induced by activated macrophages for 24 h (scale bar, 1 mm). C) Quantitative analysis of longitudinal migration of RSC96 cells. D) Schematic illustration of RSC96 cells treatment with conditioned culture media. E) Representative images from wound healing assays for RSC96 cells migration at 0, 12, and 24 h (scale bar, 400 µm). F) Quantitative analysis of the healing rates. G) Expression levels of myelination‐related genes ( PMP22 , NGF , and NCAM ) in RSC96 cells after co‐culture with the polarized macrophages for 3 days. H) IF staining for cytoskeletal protein F‐actin (green), neuron‐specific protein S100β (red), and nuclear dye DAPI (blue) as visualized by LSCM (scale bar, 100 µm). n = 3 per group. Data are expressed as mean ± SD. Ns, no significance. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: EdU‐labeled cells were observed via laser scanning confocal microscopy (LSCM, Olympus, Japan).

Techniques: Membrane, Co-Culture Assay, Migration, Expressing, Staining